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A Novel Approach to
Combat Amebiasis:
Testing Natural
Solutions on
Paramecium
Multimicronucleatum
Chris Trinidad
Trinidad 2
Purpose
The purpose of this project is to compare the
effect of natural remedies on the viability
percentage of
Paramecium
multimicronucleatum
.
Trinidad 3
Hypothesis
If sulfur and
Cinchona
alkaloids are
associated with the extermination of protozoa,
then the combination of quinine and garlic
will have the greatest effect on the viability
percentages because the sulfur of the garlic
and the anti-protozoal attributes of the quinine
will exterminate the organisms.
Trinidad 4
Materials
BSL-1 Lab
Blender
Refrigerator
Gram scale
Garlic extract ingredients/materials:
1.
24 g of peeled garlic cloves
2.
Coffee filter
3.
Glass jar
Ginger extract ingredients/materials:
1.
24 g of peeled ginger
2.
Coffee filter
3.
Knife
4.
Glass jar
Quinine extract/tonic water ingredients:
1.
28 g of chopped
Cinchona
bark
2.
2 glass jars
3.
Large glass pitcher
4.
Coffee filter
mL measuring cup
Camera
Stereoscope, if necessary
20 plastic petri dishes – 60mm x 15mm
200 individual organisms from Carolina Biological
Supply:
Paramecium multimicronucleatum
(Paramecium, Living)
Trinidad 5
1-gallon jug of Carolina Biological Springwater
(Media)
: 1
gallon is equal to 3.8 liters.
8 pipettes
4 graduated cylinders in mL
1 box of medical-grade gloves
Lab aprons (one apron for each person
experimenting)
Safety goggles (one goggle for each person
experimenting)
1 roll of paper towels
10% bleach solution – one-gallon jug: 1 gallon is
equal to 3.8 liters.
Fine tip Sharpie or permanent marker
3M masking tape
Sticky note
1 piece of paper
1 large bucket
Hydrion Spectral pH Paper Dispensers (pH 1.0 to
14.0)
Trinidad 6
Procedure
1.
Adult supervision will be maintained throughout the experiment and
appropriate lab procedures will be adhered to. Proper handling and disposal of
the
Paramecium
will be demonstrated in the experiment. A designated
supervisor and parent will be present throughout the whole duration of the
experiment. Experimenters will be using gloves, aprons, and safety goggles.
2.
Aerate culture upon delivery as soon as possible. This will be done by adult
sponsor or designated supervisor.
1.
Put on gloves, aprons, and goggles.
2.
Wipe laboratory counters with the bleach solution using paper towels.
Throw paper towels away after use in the garbage.
3.
Unscrew the lids of each of the cultures.
4.
Aerate the culture to help replace the oxygen depleted during
shipping. To do this, use a plastic pipette. Place the pipette tip into the
culture water and squeeze the bulb, bubbling air into the water.
Withdraw the pipette and release the bulb, allowing it to refill with air.
Repeat 4 more times.
5.
Replace the lids, but do not screw tight. Leave it in an area that is
around 22º Celsius.
3.
Make extracts at home, prior to laboratory work. This will be done by a
parent.
1.
Tonic water/quinine extract
1.
Measure the 28 grams of
Cinchona
bark using the
scale, then combine with the 675 mL of spring water
in a sterilized, lidded glass jar. Use the mL measuring
cup for the spring water. Shake to combine.
Refrigerate for 72 hours, shaking occasionally, at least
once per day.
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