11 of 11 What volume (in uL to 1 decimal place) of stock SybrSafe solution (3.4 mg/mL) would you add to 120 mL of agarose solution to give a final concentration of 0.4 pg/mL? Answer:
Q: A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB…
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A: We have given [ C2 H4 O3 = 1.00 , Volume= 4.00mL, pH=4.0
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A: Bradford method of protein assay is a method used for the determination of the concentration of…
Q: Standard Curve 1.4 1.2 y = 12.235x R = 0.97758 0.8 0.6 0.4 0.2 0.02 0.04 0.06 0.08 0.1 0.12 BSA (mg)…
A: In the given question, we are given a straight line graph between Absorbance at 595 nm versus BSA…
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Q: If you were presented with 2 L of a 2 M sucrose stock solution, how many grams of sugar would be in…
A: Molarity: The moles of a solute per liter of a solution is called as molarity (M).
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Q: Using 25 µL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ul
A: Firstly, we convert the 25ul into ml . i.e. 25 ul = 0.03 ml Then, there will be the multiplication…
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A: We determine the NaCl (grams) needed: 15x10-3 mol/1000 mL x500 mL x58.433 g/1 mol = 0.438 g…
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A: The unknown concentration of a substance in the given sample can be estimated by comparing the…
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Q: 1.What is the molar concentration of a 15% glycerol solution?…
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Q: 5. A 0.8720 g sample consisting solely of sodium bromide and potassium bromide yields 1.505 g of…
A: NaBr is sodium bromide KBr is potassium bromide AgBr is silver bromide Molecular weight, Na=23…
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Q: How many grams of CuSO4 would you use to make 1 L of an 8 g/L CuSO4 solution?
A: Given Values: Volume = 1 L Concentration of CuSO4 = 8 gL
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Q: BSA (mg/ml) Absorbancy 540nm 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 0.455 7 0.510 8 0.530…
A: The determination of protein concentration is a fundamental technique in biochemical analysis.
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A: Molarity is defined as the number of moles dissolves in 1000 ml of the solution.
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A: Here molar mass of MgSOu = 120.36 g/mol NaOH = 40 g/mol Ch3 oh = 32 g/mol
Q: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 2 0.420 3 0.510 4 0.610 0.720 6 0.810 7 0.915 8 0.950…
A: The Bradford Assay is is a sensitive assay to detect and quantify protein in solution.
Q: How many grams of HCl are needed to make 10 uL of a 0.005g/mL acid solution?
A: Given Values: Volume of HCl = 10 μl Concentration of HCl = 0.005 gml
Q: f there is a stock solution of 2000 (ug/ml) , is it possible to use SERIAL DILUTION to generate the…
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Q: How many grams of sucrose (molecular weight = 342 g mole−1) would you dissolve in water to make a…
A: Molarity = no. of moles of solute / liters of the solution
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Q: what is the is the dilution factor when you add 2 mL ssample of 8 mL diluent?
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Q: An IV of 650cc of 5% dextrose contains how many grams of 5% dextrose?
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Q: 950 mL of 1X CuSO₄ * 5H₂O solution from 25X CuSO₄ * 5H₂O solution
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Q: What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to…
A: We are given, [C2 H4 O3] = 1.00 M, Volume= 400.0 mL, pH = 4.00
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- After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.Siturul alORK ING )A mother brings her 251b child Into the ER with q very high fevero she has already ad ministered l2 4sp of Tylenol at home. She has brought the bottle with her and the label states: Acetamaino phere syrmp 325 mg / 5mLo Administer 10-15 mg /89 every 4 hrs as needed . How many milligrams oL Tylenot has she givern? Was this asafe dose to give the child?Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…
- AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.Your 67 YO 195lb, 5ft 10in male patient diagnosed with testicular cancer has been prescribed ifosfamide 1200mg/M2/day IV for 5 days. You calculate the BSA using the mosteller method as being 2.09m2. Ifosfamide is available in 60mL vials contianing 3g of drug. a) How many mL will you administer each day?
- Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL XA 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…
- Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.What is the amount of dextrose in mg/kg/min provided by the following TPN for a 60 kg patient? Round to 1 decimal place, do not enter units Dextrose (70%) 320 g Amino acids (8.5%) 92 g Lipids (10%) 44 g sodium chloride 60 mEq sodium acetate 40 mEq Sodium phosphate 15 mmol potassium chloride 80 mEq potassium acetate 30 mEq calcium gluconate 18 mEq magnesium sulfate 8 mEq Multivitamins 10 mL Trace elements 1 mL Total volume: 2640 mL Rate: 110 mL/hrKCI for injection contains 40 mEq in 20 mL. An order requires 15 mEq, how many mL are needed?