3. a) Briefly describe the advantage of using cardiac troponin I over cardiac troponin T in diagnosis of myocardial infarction (a heart attack). b) Enumerate five (05) features of an ideal tumour marker c) List five (05) components each for the following in a urine full report i. Macroscopic analysis of urine II. Microscopic analysis of urine 4. a) Briefly describe how the van den Burgh test is used in assessing serum bilirubin. b) List five (05) biochemical tests used in assessing renal /kidney function. c) Calculate the GFR based on the following biochemical data. 24-hour total urine volume = 1440 ml Serum creatinine concentration = 4 mg/dL == Urine creatinine concentration = 40mg/dL 3
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- Carbachol causes contractions the isolated rat ileum to contract. The responses obtained to increasing concentrations of carbachol are tabulated below. Graph the data as log concentration versus % maximum response, using Excel or another spreadsheet program. Use the graph to estimate the EC50 value for carbachol and indicate its position on the graph. Conc (M) Exp 1 Exp 2 Exp 3 (% max response) (% max response) (% max response) 1.00E-09 3.00E-09 10 20 1.00E-08 45 35 48 3.00E-08 57 69 72 1.00E-07 74 81 91 3.00E-07 95 85 96 1.00E-06 100 100 99 3.00E-06 100 98 991.Which of the following correlations about the analyses of iodine and saponificationnumbers is/are incorrect?a. Alcoholic KOH: dissolve KOH to lipid sampleb. Hanus reagent: react with saturated lipid bondc. Reflux set-up: hasten reactiond. Blank titration: determine total mol of KOH2. A triacylglycerol (Sample X) was subjected to saponification analysis. 1.1 grams of thesample was refluxed with 5% alcoholic KOH and the refluxed solution was titrated with 17.2 mLof 0.44 M HCl titrant. The blank solution was titrated with 25.6 mL of the titrant.i. What is the saponification number of the sample? ii. Which of the following could be the identity of the sample? Show solutions pls a. Tripalmitin (MW: 807.3)b. Trilaurin (MW: 639.8)c. Triolein (MW: 885:4)d. Tristearin (MW: 891.48)The measurement of LDH in the serum can be used to diagnose whether tissue damage hasoccurred.Students were tasked with the development of an assay for LDH activity in human serum samplesfrom a control patient (healthy) and a patient who had suffered cardiac damage. Two samples wereprovided, labelled C and D (Control and Diseased serum).The students decided to follow the loss of absorbance of NADH in an appropriate assay buffercontaining pyruvate as the substrate. Q1 (a) By examining the absorbance spectra(in the image attached) of both NAD+ and NADH below, comment why this approach is suitable strategy. Q1 (b). What would be the optimum wavelength at which they should measure absorbance? Give a reason for your answer. The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values…
- ing 0.03% w/w_of the same drug, who would be the percentage strength (wh) a of of the mixture? 23. A physician prescribes an suspension to contain 100 mg of corti- at n ophthalmic st sone acetate in 8 mL of normal saline solution. The pharmacist has on hand a (2.5% suspension of cortisone acetate in normal saline solution. How many milliliters of this and how many milli- le S liters of normal saline solution should b be used in preparing the prescribed sus- pension?gorq ww.Ps-3/9rie 24. R Benzalkonium ChlordaH4)ConsiteR the following Beaction: Pa,t 8 H,0→ Hy POqt 3 HCl IP 12.49 of | 10.09 of Hy O, what is the limiting Ctrl PCI, is PeacteD witn T0.0g Peactant ? Molar nhass of Pelg: 137.329/mel. holarmass of HoO=18:0291 mel malar mass of Hy POz =¢2.00 glmol molar mass of HCl= 35.4A16 glmel. %3Dh) i) j) Ph + НО OSI(CH3)3 Br PPH3 1) "BuLi /THF/rt / 1.5 h H CH3 homo /rt / 1 h 2) -Si(CH3)3 OCH3 TiCl4 CH₂Cl₂/-78°C / 15 min PPh3/1₂ (1 eq) imidazole / THE 0°C-it
- 2) You are tasked with determining the barium content of a sample containing barium nitrate mixed with rubidium nitrate. The sample is weighed, dissolved into 50.0mg water, and treated with xs 0.500M sodium sulfate. The acid fully ionizes in this experiment. A white precipitate forms which is washed, filtered, and weighed multiple times, according to the data given below: Mass of sample 0.425g Mass of thoroughly dried filter paper 1.462g Mass of precipitate+filter after 1st drying 1.755g Mass of precipitate+filter after 2nd drying 1.699g Mass of precipitate+filter after 3rd drying 1.698gA phlebotomist was acting as an instructor for a phlebotomy student from the local college and was anxious to help her learn all the special tests that had not been practiced in class. When a stat blood culture and electrolytes were ordered on a patient in the ICU, the instructor quickly grabbed the student and they headed for the floor. It was clear after looking at the patient that they would have to use a butterfly in a hand vein to collect the test specimen. The instructor was busy helping the student prepare the site and did not pay attention to the order the student layout all of the equipment/tubes/bottles for the draw. The student elated that she was able to access the difficult vein and that blood was flowing freely to the anaerobic bottles. After filling both it and the aerobic bottle to the proper level, she proceeded to draw a sst tube for the electrolytes. The blood flow was now very slow, with blood entering the tube one drop at a time. After patiently waiting for the…Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.
- Help blic Health Ch x 100% 47 HSC 258 - Major Projec X MindTap - Cengage Lea x C The Illustration To- 55750828934189288909969212&elSBN=9781305657571&id%3D1061392007&nbld%321... Q Search References Use the References to access important values if needed for this question. For the following reaction, 65.6 grams of barium hydroxide are allowed to react with 40.9 grams of sulfuric acid. barium hydroxide (aq) + sulfuric acid (aq) >barium sulfate (s) + water (1) What is the maximum amount of barium sulfate that can be formed? grams What is the FORMULA for the limiting reagent? grams What amount of the excess reagent remains after the reaction is complete? Submit AnswerThe portions of the sample having the analyte with similar composition analyzed using the same procedure at the same time, termed: O matrix O representative O assay O replicatesIn a clean sheet of paper, tabulate the differences of the Four (4) Phases of Clinical Trials by providing the following:A. DefinitionB. Primary Purpose (example: To assess safety, efficacy, etc)C. Subjects (example: healthy human subjects or human subjects with a particular disease, etc)D. Number of subjects ( example: 100, 1000, 10000, etc)