A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter).i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample.ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample.iii) Calculate the purity of the glycogen used in the sample as% Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100iv) state your answer in a complete sentence.

Given, Volume of sample of glycogen = 1 mL Moles of glucose = 35 micromole HCl volume = 2mL…

Answered: A 1 mL sample of glycogen was…

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter22: Proteins

Section: Chapter Questions

Problem 22.100P

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A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H₂O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter).

i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample.
ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample.

iii) Calculate the purity of the glycogen used in the sample as

% Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100

iv) state your answer in a complete sentence.

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For the percent purity section should the 35 be converted into moles of calculated glucose as it is currently in micromoles

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