If the biochemist used SDS-Polyacrylamide Gel Electrophoresis , predict the correct order of migration in the gel.

Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter23: Organic Polymers, Natural And Synthetic
Section: Chapter Questions
Problem 44QAP: A 1.00-mg sample of a pure protein yielded on hydrolysis 0.0165 mg of leucine and 0.0248 mg of...
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QUESTION 23
A biochemist is attempting to isolate Protein A from other proteins in solution. Only two other proteins (Proteins B and C) are present. The proteins
have the following properties:
Isoelectric
point (pl)
Molar Mass
|(kDa)
Protein
A
B
4.2
5.0
8.0
40
70
54
If the biochemist used SDS-Polyacrylamide Gel Electrophoresis , predict the correct order of migration in the gel.
O Protein C will travel the gel the fastest, followed by protein B and protein A would be the slowest.
Protein A will travel the gel the fastest, followed by protein B and protein C would be the slowest.
Protein B will travel the gel the fastest, followed by protein C and protein A would be the slowest.
Protein A will travel the gel the fastest, followed by protein C and protein B would be the slowest.
O Protein A will travel the gel the fastest, followed by protein B and protein C would be the slowest.
Transcribed Image Text:QUESTION 23 A biochemist is attempting to isolate Protein A from other proteins in solution. Only two other proteins (Proteins B and C) are present. The proteins have the following properties: Isoelectric point (pl) Molar Mass |(kDa) Protein A B 4.2 5.0 8.0 40 70 54 If the biochemist used SDS-Polyacrylamide Gel Electrophoresis , predict the correct order of migration in the gel. O Protein C will travel the gel the fastest, followed by protein B and protein A would be the slowest. Protein A will travel the gel the fastest, followed by protein B and protein C would be the slowest. Protein B will travel the gel the fastest, followed by protein C and protein A would be the slowest. Protein A will travel the gel the fastest, followed by protein C and protein B would be the slowest. O Protein A will travel the gel the fastest, followed by protein B and protein C would be the slowest.
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