Prepare 100 μL of 10.0 mM hydrolyzed sucrose in 10.0 mM acetate pH 4.8 as a standard, in a 1.5 mL microcentrifuge tube, also incorporating 5.00 μL of 5.0 mM tris pH 7.4 within the 100 μL volume. Using a stock solution of 20.0 mM sucrose substrate in 10.0 mM acetate, calculate the volumes of hydrolyzed sucrose, acetate, and dH2O.

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter10: Effect Of Electrolytes On Chemical Equilibria
Section: Chapter Questions
Problem 10.8QAP
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Prepare 100 μL of 10.0 mM hydrolyzed sucrose in 10.0 mM acetate pH 4.8 as a standard, in a 1.5 mL microcentrifuge tube, also incorporating 5.00 μL of 5.0 mM tris pH 7.4 within the 100 μL volume. Using a stock solution of 20.0 mM sucrose substrate in 10.0 mM acetate, calculate the volumes of hydrolyzed sucrose, acetate, and dH2O.

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