Three large proteins are ionized at the pH at which an electrical FFF separation is carried out. (i) if the ions are designated A2+, B2+, and C3+, predict the order of elution. (ii) What is FFF? (iii) List the four subtechniques of FFF.

Three large proteins are ionized at the pH at which an electrical FFF separation is carried out. (i) if the ions are designated A2+, B2+, and C3+, predict the order of elution. (ii) What is FFF? (iii) List the four subtechniques of FFF.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter23: Potentiometry

Section: Chapter Questions

Problem 23.11QAP

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Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.

Question

Three large proteins are ionized at the pH at which an electrical FFF separation is carried out.

(i) if the ions are designated A²⁺, B²⁺, and C³⁺, predict the order of elution.

(ii) What is FFF?

(iii) List the four subtechniques of FFF.

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