Part A Consider the proposed mechanism of an acid catalyzed aldol reaction. Mark with the green check to indicate that the given direction of electron flow using curved arrow notation is correct and with the red X label to indicate it is incorrect for this specific mechanism. Drag the appropriate labels to their respective targets. X Н. H H H H H :O: :0; H H :O: : OH H Reset Help
Q: Frog muscle cells and the solution bathing the cells contain ions at different concentrations. The…
A: The concentrations of Na+, Ca2+, K+, and Cl- are shown in the following table. Temperature = 25…
Q: What does -log(p-value) tell in immunoprecipitation mass spectrometry? What does log2 Fold change…
A: The question is asking about the interpretation of the -log(p-value) and log2 Fold Change in the…
Q: Which of the following statements regarding DNA is false? Features a beta glycosidic bond between…
A: DNA or deoxyribonucleic acid is a genetic material that transfers genetic information from one…
Q: A paste of starch was tested using Benedict’s test, the Iodine Test, and Fehling’s Test. Before…
A: There are four types of biological macromolecules- nucleic acid, proteins, lipids and…
Q: The Bohr effect describes the: cooperativity of hemoglobin in the presence of CO. effect of hydrogen…
A: A protein called haemoglobin contains iron and helps red blood cells carry oxygen. The blood's…
Q: In the case of normal RNase A, Anfisen found that oxidizing the Cys residues before slowly removing…
A: Anfinsen was trying to figure out the mechanism of folding of polypeptide chains into their…
Q: Is Lobry Van Bruyn Transformation the same as the Tautomerization of Fructose during Fehling’s test?…
A: The tautomerization process is crucial because it converts the ketone group of the ketose into a…
Q: Using proper convention, provide the amino acid sequence for the following peptide. O H3N-CH-C- CH₂…
A: For a protein, its primary structure refers to its amino acid sequence. As per the general rule, the…
Q: 4. Two common buffers in biological research are PBS and TBS. What are the compositions of these…
A: Hello! Thank you for the question. Due to time constraints, we will be able to provide the solution…
Q: The beta pleated sheet is one of the motifs for a secondary structure. Describe what this motif…
A: Proteins play an essential role in our body. They are made up of different types of amino acids. The…
Q: Calculate the change in Gibbs free energy for transport of Ca2+ from outside to inside the cell. The…
A: Free energy change (ΔG) of uncharged solute transport across a biological membrane can be calculated…
Q: Assume that the complete combustion of one mole of glucose to carbon dioxide and water liberates…
A: Given:Complete combustion of one mole of glucose liberates 2870 kJ.ΔG° = -2870 kJ/mol Efficiency of…
Q: Choose the TRUE statement. A phospholipid micelle (not a liposome) can be characterized as Forming…
A: Phospholipids are important components of cell membranes and can form various structures in aqueous…
Q: Which of the following glucocorticoids is hydrocortisone and expected to have the LEAST binding…
A: Gluco-corticoids belong to the class of corticosteroids. They belong to the class of steroids. They…
Q: ?What is the unique feature of the collagen helix compared to the a helix Hydrophobic interactions a…
A: There are four classes of biological macromolecules: proteins, nucleic acids, lipids,…
Q: Which of the following are characteristics of isoprenoid lipids? double bonds are mostly cis. methyl…
A: A type of organic molecules known as isoprenoid lipids is made up of two or more hydrocarbon units,…
Q: Question 9 Use the following information to answer Questions 9 and Part c.Consider the reduction of…
A: Stereoisomers are isomers that have the same molecular formula, same connectivity of atoms but…
Q: Which of the following statements is WRONG with regard to the following molecule? HO 0² N OH NH₂ N…
A: The given compound is cAMP. Cyclic adenosine monophosphate is a second messenger, which is important…
Q: Which of the following is characteristic of competitive inhibitors? the inhibitor could bind to the…
A: Inhibitors can be broadly classified into reversible and irreversible inhibitors. Reversible…
Q: Positive results for Benedict’s test range from green, yellow orange to red. How does the sugar…
A: There are 4 classes of biological macromolecules: nucleic acid, proteins, lipids and…
Q: The approximate composition of coconut oil (mp 24-25 °C) is listed below (data according to…
A: The biochemical composition of coconut oil consists primarily of triglycerides, which are esters…
Q: How is it possible to determine the structure of an enzyme substrate complex by x ray…
A: The objective of the question is to understand how the structure of an enzyme-substrate complex can…
Q: Construct and name the structure of a monoglyceride Two diglycerides (positions 1,2 and 1,3) and two…
A: Monoglycerides are composed of a glycerol molecule ester linked to one fatty acidDiglycerides are…
Q: Consider the structure below, and select all of the features that apply. (This is a "multi-select"…
A: Carbohydrates are polyhydroxy aldehydes or ketones. They can be classified as monosaccharides,…
Q: Match each characteristic to the appropriate process. Anabolic process breaks down macromolecules…
A: Metabolism is the sum total of all chemical reactions taking place in lifeforms. It can be divided…
Q: For a reaction that proceeds spontaneously from reactants to products, the AG is O less than 0 00 O…
A: Gibbs Free Energy is a thermodynamic function that not only is related to spontaneity but also deals…
Q: Sort the descriptions into properties that describe either saturated phospholipids or unsaturated…
A: There are four types of biological macromolecules- nucleic acid, proteins, lipids and…
Q: Using the graph paper provided, plot the velocity of the reaction (v) against the concentration of…
A: Michaelis Menten (MM) plot has [S] as X-axis and V as Y-axis. Here we are given the 1/[S] and 1/V…
Q: Why is malonate not a substrate for succinate dehydrogenase? Malonate has two carboxylic acid…
A: The reaction catalyzed by succinate dehydrogenase is given below.Succinate + FAD Fumarate + FADH2In…
Q: Can you explain the various options and how did you come to the answer? How to determine when…
A: Weak acids are the acids that do not completely dissociate into their constituent ions when…
Q: [AktivGrid] Draw the ketone body formed in ketogenesis from the condensation of two acetyl CoA…
A: Acetyl-CoA is a molecule that plays an important role in many biochemical reactions in protein,…
Q: What are ways in which cells can regenerate cytosolic NAD+? Conversion of Lactate to Pyruvate…
A: The coenzyme NAD+ is the most important oxidising reducing agent in the cell. It acts as electron…
Q: 5. ANSWER THE FOLLOWING PROBLEM AND EXPLAIN YOUR ANSWER FOR BETTER UNDERSTANDING. Fill in the blanks…
A: Amino acids are biomolecules that have a hydrogen, a carboxyl group, an amino group and a chemically…
Q: Click the "draw structure" button to launch the drawing utility. Draw the structure of the…
A: Deoxyribonucleotide is a structure that is major component of DNA. It is made up of three parts.…
Q: Decide whether each molecule in the table below could be found embedded in the outer surface of a…
A: Cell membranes are mostly composed of phospholipids. An important characteristic of phospholipids is…
Q: 1. What happens to electrons in an oxidation reaction? Electrons are shared equally. Electrons are…
A: Note: since you have posted multiple questions we will solve first three questions for you as per…
Q: Why is it not to the cell's advantage to simply make more NAD so that the regeneration would not be…
A: NAD is an electron carrier. It carries electrons from substrate oxidation to the ETC to generate a…
Q: Congratulations! Due to your success in Dr. Daniel's Cellular Metabolism class. You have been…
A: A reaction is said to be an oxidation reaction if it remove electrons. In other words, the oxidation…
Q: The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. If…
A: Inhibition can be either reversible or irreversible. Reversible inhibition can be generally of 3…
Q: Which of these molecules is most likely to interact with an intracellular receptor? О HO НО H₂N ОН…
A: The cellular receptors are involved in signal transduction processes.Cell surface or membrane…
Q: create a table to summarize the ATP count for the listed carbohydrates below that will undergo…
A: The difference between the aerobic breakdown of sugars in liver, kidney & heart v/s muscle &…
Q: Give an overview for the oxidative breakdown of a six-carbon, saturated fatty acid. Decide how the…
A: The 6-carbon fatty acid will undergo two rounds of beta-oxidation to produce three molecules of…
Q: 2 SCOA 1) fatty acid synthase (1 cycle) 2) NADH reductase all in water
A: The image shows a chemical reaction. The reactants are fatty acid synthase (1cycle) and NADH…
Q: We would be having a lab activity about carbohydrates. I would like to ask the following to compare…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Class of lipid Function(s) of the class Ester of what fatty acid?? Why is this molecule considered…
A: Lipids are a chemically diverse group of biomolecules that have two things in common: low solubility…
Q: what concentration of a competitive inhibitor is required to yield 60% inhibition of an enzyme at a…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: A. Provide a reasonable mechanism for the production of geranyl pyrophosphate from IPP and DMAPP B.…
A: The pyrophosphate ester of the terpenoid geraniol is geranyl pyrophosphate (GPP), often referred to…
Q: Metabolizing a fatty acid via the B-oxidation pathway will generate acetyl CoA, NADH and FADH2.…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbons to 36 carbons.…
Q: An enzyme has a Km of 10 mM and a Vm of 100 mmol/min If [S]=10 mM, which will increase the velocity…
A: For a one-substrate enzyme catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: Page 17 Use the Michaelis-Menten equation to calculate the missing values of [S] given below if Vmax…
A: In order to find the different substrate concentrations, first we need to find the Km value. Given…
8L.4.2
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 2 images
- There is another class of aldolase enzymes known as Class II. These enzymes are found in fungi, algae, and some bacteria. This class differs for Class I in that these enzymes do not have a Lys residue associated with their active sites, but contain a divalent cation (usually Zn2+ or Fe2+) in the active site. Outline a possible mechanism for a Class II aldolase and explain the function of the metal ion in the reaction.Which of the following statements is true about the chymotrypsin reaction? 1. The catalytic triad at the active site is formed by three residues: His, Gly, and Ser 2. When the substrate is p-Nitrophenylacetate, the rate-limiting step is the release of p-Nitrophenolate. 3. During the reaction, LBHB forms when the proton is donated to His from Ser or water. 4. The chymotrypsin reaction involves “only” specific acid-base catalysis 5. The “oxyanion hole” is formed by the amide nitrogens of Ser and GluDirections: Solve the following problem: The enzyme ẞ-methylaspartase catalyzes the deamination of ẞ-methylaspartate: CH,NH, CH OOC-CH-CH-COOOOC-CH-CH-COO+NH mesaconate Williams and Selbin The effects of hydroxymethylaspartate as an inhibitor for this enzyme was studied. The following data wer Substrate Concentration obtained: Reaction Rate without inhibitor (mM) 1 × 10-4 5 x 10-4 1.5 x 10-3 2.5 x 10-3 5 x 10-3 (mM/s) 0.026 0.092 0.136 0.150 0.165 Reaction Rate with inhibitor (mM/s) 0.010 0.040 0.086 0.120 0.142 Use Lineweaver-Burk plot to determine the KM and Vmax of the enzyme in the absence of inhibitor. Moreover, determine as well whether the inhibitor is competitive or noncompetitive. Show the graphs and calculations below.
- Propose a mechanism for the Co2 + catalyzed hydrolysis of glycinamide.Describe the two phases of the the HMP shunt. Enumerate the reactions in each phase and the corresponding enzyme of each reation. Explain how the HMP shunt is regulated?Bypass reaction in the anabolic pathway often occurs when A The ΔG of the reverse reaction in the catabolic pathway has a large negative value B The ΔG of the reverse reaction in the catabolic pathway has a large positive value C The ΔG of the reaction in the anabolic pathway is close to zero D The ΔG of the reaction in the anabolic pathway is positive E None of the above
- Figure I shows the Michaelis Menten plot of initial reaction velocity (as percentage of Vmax) versus [S] (concentration) for the carbonic anhydrase reaction in the absence and presence of the inhibitor acetazolamide. Carbonic anhydrase participates in regulation of the pH and bicarbonate content of a number of body fluids. 100 No inhibitor Acetazolamide 0.2 0.4 0.6 0.8 1 [S] (mM) Figure 1 (i) Compare Vmax and Km of the enzyme without inhibitor and in the presence of acetazolamide. Determine the type of inhibition shown by acetazolamide. Explain your answer. (ii) Name TWO (2) other types of inhibitions besides the inhibition shown by acetazolamide in Qla)(i). List down the kinetic properties of these inhibitions. Sketch a graph of I/V versus 1/[S] showing plots in the absence of an inhibitor and in the presence of the types of inhibitors mentioned in Qla)(ii). (iii) V (% of VmaxThe table below shows the purification of liver lactate dehydrogenase. Purification Table of liver LDH Step Crude Extract Ammonium Sulfate DEAE-Sephadex 5'AMP-Sepharose Volume (mL) 200 100 112 40 LDH activity (Units/mL) (mg/mL) [Protein] 47.0 50.00 90.0 63.00 51.0 1.50 137.0/ 0.4 Calculate the LDH yield for the ammonium sulfate step. Provide answer to one decimal only. Show Transcribed Text this is biochemistry and the topic is protein purification could you pleaae show me the full workings even the workings for the comversionsAcetazolamide is a drug which inhibits carbonic anhydrase. Carbonic anhydrase participates in regulation of the pH and bicarbonate content of a number of body fluids. Figure 2 shows the experimental curve of initial reaction velocity (as percentage of Vma) versus [S] (concentration) for the carbonic anhydrase reaction. The graph also shows the curve in the presence of acetazolamide. 100 No inhibitor 50 Acetazolamide 0.2 0.4 0.6 0.8 [S] (mM) Figure 2 (i) Compare the maximal velocities and Michaelis Menten constants of the enzyme in the absence and the presence of the inhibitor acetazolamide. Determine the nature of inhibition by acetazolamide. Explain your answer. (ii) Name TWO (2) other types of inhibitions besides the inhibition shown by acetazolamide. Sketch a graph of V versus [S] showing curves in the absence of an inhibitor and in the presence of the types of inhibitors not shown by acetazolamide. ("AJO %) A
- a) Calculate the enzyme and specific activity of a reaction with 3 μM Hsp90 using the following information: The rate is measured in a spectrophotometer as 0.028 OD units/min in a 1 ml reaction volume. The absorbance was detected at 340nm and the extinction coefficient for NADH at this wavelength is 6200 L M-1 min-1 and the molecular mass of Hsp90 is 82.7 kDa. The rate of NADH utilisation is equivalent to the rate of ATP utilised by Hsp90. Show all your calculations and the units for your answers. b) Calculate the turnover number for the reaction described in (a) aboveWhich of these heterocyclic drugs is likely to be the least soluble in water? Use the Fsp³ parameter to decide. OH Tramadol Chemical Formula: C16H25NO2 YOUR OW Pantoprazole Torasemide Chemical Formula: C16H15F2N3O4S Chemical Formula: C16H20N4O3S Temazepam -OH Chemical Formula: C16H13CIN₂O2 Tioconazole Chemical Formula: C16H13C3N₂OS A. Tramadol B. Pantoprazole C. Torasemide D. Temazepam E. Toconazolea) Calculate the enzyme and specific activity of a reaction with 3 pM Hsp90 using the following information: The rate is measured in a spectrophotometer as 0.028 OD units/min in a 1 ml reaction volume. The absorbance was detected at 340nm and the extinction coefficient for NADH at this wavelength is 6200L M- 1 min-1 and the molecular mass of Hsp90 is 82.7 kDa. The rate of NADH utilisation is equivalent to the rate of ATP utilised by Hsp90. Show all your calculations and the units for your answers. b) Calculate the turnover number for the reaction described in (a) above
